Photometer for Reducing Sugars in Wine - HI83746-01

Sugar is an essential component in the production of wine. During alcoholic fermentation, yeast consume sugars found in the grape juice, or must, and converts it to ethyl alcohol and carbon dioxide. In the case of certain styles of wine such as semi-sweet or dessert wines, some sugar is allowed to remain post-fermentation. This residual sugar can serve to provide a sweeter character to the final blend or play a role in microbial stability.

The primary fermentable sugars found in grapes are glucose and fructose. These two simple sugars are also known as reducing sugars because they contain functional groups capable of being oxidized under certain conditions. After reaction with excess alkaline cupric tartrate (Fehling reagents), the content of reducing sugars can be determined colorimetrically. The Fehling method is not an exact determination but an index of the reducing sugar concentration, because the reaction depends upon the amount and type of reducing sugars present. When the reducing sugar content is known at the beginning of fermentation, the potential alcohol degree can be estimated by multiplying the sugar concentration (in g/L) by 0.06.

The HI83746 uses the Fehling method to determine the concentration of reducing sugars less than 50.00 g/L (ppt). When Fehling’s A and Fehling’s B Solutions react with a sample containing reducing sugars, the sample will undergo a color change; the greater the concentration, the deeper the color. The associated color change is then colorimetrically analyzed according to the Beer-Lambert Law. This principle states that light is absorbed by a complementary color, and the emitted radiation is dependent upon concentration. For determination of reducing sugars, a narrow band interference filter at 610 nm (orange) allows only orange light to be detected by the silicon photodetector and omits all other visible light emitted from the tungsten lamp. As the change in color of the reacted sample increases, absorbance of the specific wavelength of light also increases, while transmittance decreases.